Comparison of the Effectiveness of Maceration, Digestion, and Soxhlet Methods on the Concentration Value of Cassava Leaf (Manihot esculenta Crantz) Extract Stock Solution


crossmark

Authors

  • Putri Ananda Nieyan Program Studi Rekayasa Kosmetik, Fakultas Teknologi dan Industri, Institut Teknologi Sumatera, Lampung Selatan, Lampung
  • Salsabila Sofia Hakiki Program Studi Rekayasa Kosmetik, Fakultas Teknologi dan Industri, Institut Teknologi Sumatera, Lampung Selatan, Lampung
  • Audy Nasyiah Rahim Program Studi Rekayasa Kosmetik, Fakultas Teknologi dan Industri, Institut Teknologi Sumatera, Lampung Selatan, Lampung
  • Anis Azizah Program Studi Rekayasa Kosmetik, Fakultas Teknologi dan Industri, Institut Teknologi Sumatera, Lampung Selatan, Lampung
  • Salsabila Nurrahmah Program Studi Rekayasa Kosmetik, Fakultas Teknologi dan Industri, Institut Teknologi Sumatera, Lampung Selatan, Lampung
  • Herayati Program Studi Rekayasa Kosmetik, Fakultas Teknologi dan Industri, Institut Teknologi Sumatera, Lampung Selatan, Lampung
  • Iwan Syahjok Saputra Program Studi Rekayasa Kosmetik, Fakultas Teknologi dan Industri, Institut Teknologi Sumatera, Lampung Selatan, Lampung

DOI:

https://doi.org/10.69606/jcse.v2i02.263

Keywords:

Cassava Leaves, Extraction, Maceration, Digestion, Soxhlet

Abstract

Cassava leaves (Manihot esculenta Crantz) are known to contain various bioactive compounds such as flavonoids, tannins, saponins, and alkaloids. This study aimed to compare the effectiveness of three extraction methods maceration, digestion, and Soxhlet extraction in producing stock solutions of cassava leaf extract. A total of 5 grams of cassava leaf simplicia was extracted using methanol 96% (maceration), distilled water (digestion), and methanol 99.9% (Soxhlet). The extract yield was measured by evaporating 1 mL of filtrate and weighing the residue. The results showed that the Soxhlet method produced the highest concentration of 0.085%, followed by digestion (0.0132%) and maceration (0.0106%). Soxhlet extraction was more effective due to its continuous solvent circulation at elevated temperatures and higher solvent volume, resulting in a more efficient extraction of active compounds from the cassava leaves.

References

Abubakar, A. R., & Haque, M. (2020). Preparation of medicinal plants: Basic extraction and fractionation procedures for experimental purposes. Journal of Pharmacy and Bioallied Sciences, 12(1), 1-10. DOI: 10.4103/jpbs.JPBS_175_19

Ambair, I. K., Doughari, J. H., Pukuma, M. S., & Ewansiha, J. U. (2022). Percentage yield and acute oral toxicity test of Anogeissus leiocarpus crude extracts from the wild in Gulum area of Jalingo, Taraba State, Nigeria. IOSR Journal of Pharmacy and Biological Sciences (IOSR-JPBS), 17(5 Ser. I), 49–53. DOI:10.21767/2393-8862.100010

Amelework, A. B., & Bairu, M. W. (2022). Advances in genetic analysis and breeding of cassava (Manihot esculenta Crantz): A review. Plants, 11(12), 1617. DOI: 10.3390/plants11121617

Antony, A., & Farid, M. (2022). Effect of temperatures on polyphenols during extraction. Applied Sciences, 12(4), 2107. DOI: 10.3390/app12042107

Arsyad, R., Amin, A. and Waris, R. 2023. Teknik Pembuatan Dan Nilai Rendamen Simplisia Dan Ekstrak Etanol Biji Bagore (Caesalpinia Crista L.) Asal Polewali Mandar. Makassar Natural Product Journal 1(3), pp. 2023–138.

Asworo, R.Y. and Widwiastuti, H. 2023. Pengaruh Ukuran Serbuk Simplisia dan Waktu Maserasi terhadap Aktivitas Antioksidan Ekstrak Kulit Sirsak. Indonesian Journal of Pharmaceutical Education 3(2), pp. 256–263.

Čižmárová, B., Hubková, B., Tomečková, V., & Birková, A. (2023). Flavonoids as promising natural compounds in the prevention and treatment of selected skin diseases. International Journal of Molecular Sciences, 24(7), 6324. DOI: 10.3390/ijms24076324

Dewi, T. O. T., Dewi, Y. S. K., & Sholahuddin, S. 2025. Kajian Suhu Pengeringan Terhadap Karakteristik Fisikokimia Dan Sensori Pada Teh Herbal Kulit Buah Nanas (Ananas comosus (L.) Merr.). Jurnal Sains Pertanian Equator, 10(3).

Herwanto, T. and Harnesa Putri, S. 2016. Aktivitas Antioksidan Pada Minyak Biji Kelor (Moringa Oleifera L.) Dengan Metode Sokletasi Menggunakan Pelarut N-Heksan, Metanol Dan Etanol (Antioxidant Activity in Oil Seeds Moringa (Moringa oleifera l.) Soxhletation Method with using Solvents N-hexane, Methanol and Ethanol). Jurnal Teknotan 10(2). DOI: 10.24198/jt.vol10n2.3

Mahendradatta, M., & Hasizah, A. (2023, June). Comparison of soxhletation and microwave assisted extraction method for extracting polyphenols in cacao pod husks (Theobroma cacao L.). In IOP Conference Series: Earth and Environmental Science (Vol. 1200, No. 1, p. 012038). IOP Publishing. DOI: 10.1088/1755-1315/1200/1/012038

Ningsih, E. S., et al. (2022). Effect of extraction methods on yield and antioxidant activity of cassava leaves. IOP Conference Series: Earth and Environmental Science, 1107(1), 012005.

Pratiwi, R., et al. (2023). Comparison of extraction methods for bioactive compounds from medicinal plants. Heliyon, 9(3), e16666.

Priyadi, A., Harun, F. R., Daulay, A. S., & Ridwanto, R. (2025). Comparison of maseration and soxhlet extraction on the total phenolic content of ethanol extract of betel leaves (Piper betle L.) using visible spectrophotometry. Journal of Pharmaceutical and Sciences, 8(1), 550–563. https://doi.org/10.3390/molecules27051556

Saintika, J.P., Amin, A. and Waris, R. 2025. Optimasi Waktu Ekstraksi Daun Pecut Kuda (Stachytarpheta jamaicensis) Dengan Metode Digesti Dan Nilai Rendemen Ekstraknya. Journal Pharma Saintika 8(April), pp. 2–7. DOI: https://doi.org/10.51225/jps.v8i2.76

Suhendra, D., et al. (2020). Pengaruh jenis pelarut terhadap kandungan fenolik dan aktivitas antioksidan ekstrak daun kelor (Moringa oleifera). Jurnal Ilmu Pertanian Indonesia, 25(2), 93–98. DOI: 10.3350/ebio.v4i02.506

Triyanti, S. B., Lestari, F. P., Fitriana, P. A. N., Rostiana, H. R., Silalahi, D. D., Syalsabina, T. D., & Saputra, I. S. (2025). Pengaruh metode ekstraksi maserasi, sonikasi, dan sokletasi terhadap nilai rendemen sampel kulit buah naga (Hylocereus polyrhizus). Jurnal Sains dan Edukasi Sains, 8(1), 71–78. DOI: https://doi.org/10.24246/juses.v8i1p71-78

Febriyanti, T.A., Anisyah, A., & Hasana, A. R. (2024). Pengaruh perbedaan pelarut etanol 96% dan metanol pada terhadap penetapan kadar flavonoid total ekstrak polong cengkeh (Syzygium aromaticum L.). SENTRI: Jurnal Riset Ilmiah, 3 (10).

Published

2025-11-29

How to Cite

Nieyan, P. A., Hakiki, S. S., Rahim, A. N., Azizah, A., Nurrahmah, S., Herayati, & Saputra, I. S. (2025). Comparison of the Effectiveness of Maceration, Digestion, and Soxhlet Methods on the Concentration Value of Cassava Leaf (Manihot esculenta Crantz) Extract Stock Solution. Journal of Chemistry Sciences and Education, 2(02), 47–54. https://doi.org/10.69606/jcse.v2i02.263